Tincion de robert feulgen biography
Robert feulgen biography.
A brief history of the Feulgen reaction
Introduction
The year 1924 marked a significant milestone in the advancement of histocytochemical studies.
Tincion de robert feulgen biography
That was the year when Feulgen and Rossenbeck published a chemical reaction which allowed the specific staining of deoxyribonucleic acid (DNA) in histological specimens (Feulgen and Rossenbeck 1924). At that time, Watson and Crick’s studies on the structure of the DNA double helix were still far in the future and the scientific community had divergent opinions regarding the presence of DNA within cell nuclei.
Although acknowledging the studies of Miescher who had demonstrated the presence of a phosphorous-rich acid presumably present in the nuclei of purulent cells (Miescher 1871), Feulgen and Rossenbeck themselves raised questions about the actual nuclear presence of nucleic acids, recognizing the difficulties arising when isolating them from complex tissues since there was no guarantee that the extracted material actually came from